![]() ![]() We found mainly a significant effect on the gene expression of duodenal mucosa in gluten-fed broilers at 1 week (289 differentially expressed genes ). Histochemical photomicrographs showed a reduced villus height to crypt depth ratio in the duodenum of gluten-fed broilers at 1 week. A reduction in feed intake and body weight gain was found in the broilers fed a high-gluten containing diet at both ages. Transcriptomics and histochemical techniques were employed to study the effect of gluten on their duodenal mucosa using randomly selected 12 broilers (3 chicks per group). A total of 120 broilers (Ross Strain) were used to perform two animal experiments consisting of two gluten inclusion levels (0% or 25%) by bird's age (1 week or 4 weeks). This study aimed to identify effects of high-gluten diets on chicken small intestines and the variation of their associated transcriptional responses by age. Wheat gluten is an increasingly common ingredient in poultry diets but its impact on the small intestine in chicken is not fully understood. Results in each bar graph are the composite data from 3 independent experiments performed in triplicate (mean Ϯ SEM). Cells were then labeled with BrdU, 7-AAD, and lineage markers (CD11b and CD71). (C) Primary human bone marrow cells were infected with control or ribosomal gene shRNAs and allowed to grow and differentiate over 5 days in the presence of cytokines supporting erythroid and myeloid differentiation. Lineage-specific activation of p21 protein was determined by staining for erythroid (CD71) and myelomonocytic (CD11b) cell surface markers. (B) Levels of p21 were determined by intracellular flow cytometry in cells expressing control ( luciferase ), RPS14, or RPS19 shRNAs. Lineage-specific activation of p53 protein was determined by staining for erythroid (CD71), megakaryocytic (CD41a), and myelomonocytic (CD11b) cell surface markers. (A) Levels of p53 were determined by intracellular flow cytometry in cells expressing control ( luciferase ), RPS14, or RPS19 shRNAs. Lineage specificity of p53 accumulation, p21 levels, and cell cycle arrest. ![]()
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