![]() Primer-Blast analyses indicated that the primers were specific to PvSV. Then open Geneious and go to Tools->Preferences and set the data storage location to this database folder. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. The primer and probe binding regions were selected by the Geneious Prime Primer Design Algorithm considering the optimal fragment size rage of 100220 bp and an annealing temperature of 60 C. To load a database backed up with a system or third party backup tool, first transfer the database folder out of the backup drive and into your User directory. geneious format, rather than a full database backup, you can load these into your existing Geneious database by dragging and dropping them into the Geneious window. 1.1 Downloading & Installing Geneious Prime 1.2 Geneious Prime setup 1.2.1 User preferences 1.2.2 Choosing where to store your data 1.2.3 Sharing les or the local database 1.2.4 Changing the memory available to Geneious Prime 1.2.5 Connecting to the internet from within Geneious Prime 1.2. For instructions on how to combine a restored backup with your existing data, please see How do I merge a restored backup with my existing data? However the existing data has not been deleted. Note that after you restore a backup in this way the back up data will replace any existing data you had in Geneious. At the prompt where you are asked to choose a directory to restore the backup to, you must choose either an empty folder on your drive, or type the name of a new folder that does not currently exist (Geneious will then create the folder to restore the backup to).Īfter the backup is extracted, you will see the following window:Ĭlick Load Restored Data to load your backed up data into Geneious. backup.zip, you can restore this by opening Geneious and going to File -> Restore Backup.īackup.zip files are entire databases by themselves and cannot be restored into an existing Geneious database. The protocol in this study could provide rapid and efficient WGS that could be applied to study the biology of RNA viruses including identification, characterization, and global surveillance.If you have taken a backup of your Geneious database using the Geneious backup tool, and have a file with the suffix. These long-reads could make overall structure of whole genome easily, and WGS faster and easier. We also found that the maximum length of reads was 10,311 bp which is almost the whole genome size of ZIKV. The sequence identities of the ZIKV PRVABC59 and MR 766 strains for each reference genomes were 98.76% and 99.72%, respectively. The sequenced reads resulted in 99% and 100% genome coverage with 63.5X and 21,136X, for the ZIKV PRVABC59 and MR 766 strains, respectively. In this study, we obtained WGS of two strains of ZIKV following the established protocol. It makes WGS easier and faster with long-read assembly. Nanopore technology also allows to read long sequences. This established method does not require specific primers, complimentary DNA (cDNA) synthesis, and polymerase chain reaction (PCR)-based enrichment, resulting in the reduction of biases as well as of the ability to find novel RNA viruses. We established an improved method using direct RNA sequencing with Nanopore technology to obtain WGS of ZIKV, after adding poly (A) tails to viral RNA. Whole genome sequencing (WGS) of viruses could be applied to viral pathogen characterization, diagnosis, molecular surveillance, and even finding novel pathogens. Zika virus (ZIKV) is one of the pathogens which is transmitted world widely, but there are no effective drugs and vaccines. geneious prime software, geneious primer design, geneious prime license, geneious prime 2021, geneious prime citation, geneious prime manual 837fadd873. ![]()
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